A 50-year-old man in Cambodia has died from H5N1 bird flu, marking the second death from the virus in the country this year. Prior to his death about 50 chickens had died and were shared with neighbors to eat.

Cambodian authorities reported two cases of avian flu, including an 11-year-old girl who died of the virus, the UN health agency said on Sunday.

The death of birds recently discovered in Tuolporn Taley Boeung Sne protected area in Prey Veng province’s Ba Phnom district was a result of bird flu caused by influenza A virus subtype H5N1 (A/H5N1), officials confirmed. Puth Bo, community chief in the Tuolporn Taley Boeung Sne area, said community members and specialists had collected and destroyed 135 bird carcasses, raising the total to nearly 2,000 since March 17.

Cambodia has tested at least 12 people for the H5N1 strain of avian influenza, the health ministry said, after an 11-year-old girl died this week from the virus in the first known transmission to humans in the country in nearly a decade.